Recombination mapping is used to determine if two genes are linked (recombination frequency <50%) or unlinked (recombination frequency ~50%). The distance between two genes is measure in either map units (mu) or centiMorgans (cM).
Pedigrees are diagrams that chronicle the phenotype/genotype of organisms over multiple generations. They can be used to determine the mode of inheritance of a particular disease as well as determine the probability of a future offspring inheriting it.
Mutations refer to the changes in the genetic sequence that can lead to changes at the RNA and protein level. These changes can have a wide variety of consequences. In this post, I will summarize the major types of mutations critical to the field of genetics.
Biochemical pathways are straightforward. A gene is transcribed and translated into a protein that plays a catalytic role in a biochemical pathway.
Mitosis is the process by which a eukaryotic cell nucleus splits into two followed by the division of a parent cell into two daughter cells. Meiosis, on the other hand, is a type of cell division that is required to produce gametes.
The lac operon has been covered in another post along with the possible scenarios you could encounter. But what happens to B-galactosidase expression, permease expression and trans-acetylase expression if you have an extrachromosomal F’ factor episome that carries another lac operon? Well, not to fear! I will guide you through a sample problem giving you a systemic method to solve these types of problems.
Complementation testing is done to determine whether two mutants contain a mutation in the same genes or different genes. In research, it is practical to have various mutants that have mutations on the same gene to study its role in the native system. But before one jumps into using these mutants to study a particular gene of interest, one must learn the basics of complementation testing.